Amanda Grayson was a Human teacher from Earth born around the turn of the 23rd century. (TOS: "This Side of Paradise") During the early 2230s, Amanda met Sarek, the Vulcan Ambassador to Earth. The two later married, and she returned to Vulcan with Sarek. (TOS: "Amok Time")
In 2230, Amanda gave birth to her only son, Spock.
In the 2240s, Spock got into a fight with another Vulcan child when he referred to Spock's mother as a "Human whore."
In around 2250, when Spock was considering completing the kolinahr discipline, which would have purged the last vestiges of his emotions, he expressed his wish that Amanda not consider his choice to live a life free from emotion as a reflection upon her. Amanda assured her son that no matter what he chose, she would always be proud of him. Spock ultimately decided to enroll in Starfleet Academy rather than the Vulcan Science Academy after the head minister of the Vulcan Council referred to Spock's mother as a "disadvantage".
In 2258, Vulcan was attacked by the Romulan mining vessel Narada. Attempting to preserve the knowledge of the Vulcan culture, Sarek, Amanda, and the Vulcan Council went into the Katric ark, making evacuation by transporter impossible. Spock beamed down to the surface and evacuated them from the ark so that they could be beamed aboard the USS Enterprise. As the transporter was initiated, the ledge beneath Amanda collapsed, preventing a viable transporter lock, and Amanda was lost.
James T. Kirk later used Amanda's death to encourage an emotional reaction from Spock by questioning whether he ever loved her (as an eyewitness to Spock's reaction to her death, he knew this was his best chance to successfully do so), thus forcing Spock to recognize that he had been emotionally compromised by her death and was thus unsuited to command the current mission against Nero. Spock and Sarek later spoke about Spock's inability to control his anger at Nero for causing Amanda's death, prompting Sarek, knowing that suppressing such strong emotion would've been the last thing she would want Spock to do, to admit to his son that he had genuinely loved his wife, helping Spock achieve a greater control of himself. (Star Trek)
Amanda Grayson was portrayed by Winona Ryder. Additional scenes featuring Amanda were shot for the film Star Trek, but cut before the final release. One such scene features Amanda and Sarek (predating Nero's incursion, Ryder and Ben Cross were actually playing Jane Wyatt and Mark Lenard's "prime" characters), together with the newborn Spock.
Concerning Ryder's performance as Amanda Grayson, Spock actor Zachary Quinto once stated, "Winona brought a real tenderness to the role and she really highlights the juxtaposition between her heartfelt way of being and Sarek's logic."