Frankenstein is a novel written by Mary Shelley that was later adapted in 1931 into a movie. The book and film depict a scientist named Dr. Frankenstein attempting to create life by reanimating a creature composed to dead human body parts. The film featured actors Colin Clive as Henry Frankenstein, Dwight Frye as Fritz, and Boris Karloff as Frankenstein's monster. The film was followed by several sequels, including Bride of Frankenstein, Son of Frankenstein, and Abbott and Costello Meet Frankenstein.
In 2153, Tucker managed to convince Chef to show a three-night marathon of Frankenstein, Bride of Frankenstein, and Son of Frankenstein, supposedly the three greatest horror movies ever (and maybe Abbott and Costello Meet Frankenstein) while Travis Mayweather was out. He and Jonathan Archer convinced T'Pol to watch the movie, not without a great effort. Although T'Pol was initially skeptical, preferring a dramatic reading of the book, she found the movie interesting enough to shush Phlox when he was began talking about medical details during the projection. (ENT: "Horizon")
T'Pol believed that the Vulcans felt very much like Frankenstein's monster when they landed on Earth. The Humans feared them, but as Captain Archer pointed out, the townspeople didn't chase them with "torches and pitchforks". Nonetheless, T'Pol considered showing the film to Ambassador Soval and other Vulcan diplomats to understand Human psychology. As a result, Tucker and Archer began to regret pressing T'Pol into watching the film in the first place. (ENT: "Horizon")
When Wesley Crusher worked overtime on one of his science projects, nanites, he stated, that it was "just a science project." Guinan commented "A doctor friend once said the same thing to me. Frankenstein was his name." Wesley later stated that he always got an A, whereupon Guinan said "So did Doctor Frankenstein." (TNG: "Evolution")
- The TNG episode "Thine Own Self" was inspired by Frankenstein. (Star Trek: The Next Generation Companion)
- Christian Ford and Roger Soffer's story pitch that became DS9: "Life Support" was based on Frankenstein. (Star Trek: Deep Space Nine Companion)
- Sound designer Ben Burtt used a recording of the props from the film as part of the transporter sound effects in Star Trek and Star Trek Into Darkness. 
- The various "kitbashed" starships, composed of parts from other ships, seen in the background of various episodes of Star Trek: Deep Space Nine were sometimes referred to as the "Frankenstein fleet".